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2 edition of Microbiological assay of nicotinic acid in natural materials found in the catalog.

Microbiological assay of nicotinic acid in natural materials

Raymond Leroy Pederson

Microbiological assay of nicotinic acid in natural materials

by Raymond Leroy Pederson

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  • 10 Currently reading

Published .
Written in English

    Subjects:
  • Niacin.,
  • Vitamins.

  • Edition Notes

    Statementby Raymond Leroy Pederson.
    The Physical Object
    Pagination24 leaves, bound :
    Number of Pages24
    ID Numbers
    Open LibraryOL15102891M

    Assay of Pantothenic Acid served that slight deviations in the curve result on different days. Application of Method for Assay of Extracts of Natural Materials -Aqueous extracts of natural materials were prepared as pre- viously described (3, 7). The material was autoclaved for The coefficient of variation of the microbiological results was of the order of 9 yo. In a previous paper (Kodicek & Pepper, ) we examined various factors which may influence the microbiological assay of nicotinic acid, and described a medium allowing a slightly higher acid production than with formerly described media.

    Microbiological, nutritional quality and antioxidant activity of fermented Delonix regia seeds nicotinic acid, riboflavin and perhaps, protein content are enhanced as a result of microbial The colorimeter assay was used to determine the total flavonoid content in the fermented. D. regia. Barton-Wright EC, Booth RG. The assay of riboflavin in cereals and other products: 1. Microbiological assay. 2. Fluorometric assay. Biochem J. Apr; 37 (1)– [PMC free article] []Kodicek E. Estimation of nicotinic acid in animal tissues, blood and certain foodstuffs: Applications.

    An occasional acid bath, e.g., with about 2 N nitric acid or with chromic acid (see Cleaning Glass Apparatus ) is needed. Turbidimetric Assay Receptacles For assay tubes, use glass or plastic test tubes, e.g., 16 × mm or 18 × mm that are relatively uniform in length, diameter, and thickness and substantially free from surface. Niacin, also called nicotinic acid, is a B vitamin (vitamin B3). It occurs naturally in plants and animals, and is also added to many foods as a vitamin supplement. It is also present in many multiple vitamins and nutritional supplements. Other names: Nicotinic Acid, Bionic, Vitamin B3. Uses.


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Microbiological assay of nicotinic acid in natural materials by Raymond Leroy Pederson Download PDF EPUB FB2

Report on the microbiological assay of riboflavine and nicotinic acid Analytical Methods Committee, Analyst,71, DOI: /AN The chapter accounts for the microbiological assay techniques for the determination of vitamins like thiamine, pantothenic acid, nicotinic acid and amide, biotin, inositol, vitamin B 6, folic acid, folinic acid, and vitamin B 12 in body fluids.

The establishment of quantitative methods for the determination of vitamins in body fluids and Cited by: Microbiological assay of nicotinic acid in natural materials. optimum assay conditions.

\ud The procedure followed in the improvement of the assay method was to use extracts of natural materials in the basal medium provided for the growth of Lactobacillus arabinosus. These extracts were rendered nicotinic acid-free by adsorption of. The microbiological assay of antibiotics and vitamins were studied by using various strains of microbes.

of the material taken for assay. 9, Nicotinic Acid. Microbiological and Chemical Assay of Nicotinic Acid in B Complex Products Article in Industrial & Engineering Chemistry Analytical Edition 15(1) May with 5 Reads How we measure 'reads'. MICROBIOLO'3-ICAL ASSAY OF NICOTINIC ACID IN NATURAL MJTERIALS INTRODUCTION Nicotinic acid, popularly called niacin, has received widespread attention since its discovery in as the an- ti-pella5ra vitamin (6).

The vitamin occurs chemically combined as di- and tri-phosphopyridine nucleotides and al- so occurs free as nicotinamide nd nicotinic acid. MICROBIOLOGICAL ASSAY OF RIBOFLAVIN AND NICOTINIC ACID variation in the standard curves has 'been encoun- tered over a period of twelve months, such controls are essential as a check on the non-varying depen- dence of the organism for the particularvitamin under investigation and the constancy of the medium.

/ Riboflavin.-Assay tubes were prepared as follows: Blanks ml. of water and 5 ml. The Structure and Estimation of Natural Products Functionally Related to Nicotinic Acid. Chemical Reviews33 (3), DOI: /cra R. Greene, Archie Black, and F. Howland.

Microbiological and Chemical Assay of Nicotinic Acid in B Complex Products. A method of estimating niootinio acid by the growth of Lactobocillus ardbinosus is described. The medium contained per litre, 40 g. glucose, 36 g. anhydrous sodium acetate, 10 g.

hydrolysed casein, 2 g. yeast extract treated with norite, mg. cystine, µCa pantothenate, 10 ml. each of inorganic salt solutions as described by Snell and Wright (Abst.

Vol. 11), and 5 g. peptone. Theory and Application of Microbiological Assay first presents an overview of microbiological assay, including general principles, basic techniques, capabilities, and limitations.

This book also explains when to use a certain microbiological assay for specific analysis, citing examples to further clarify the matter. LEIFER et al.1 have reported the paper chromatography of nicotinic acid with the use of microbiological assay.

While our work was in progress, Huebner2 described a spot test for detection of. The microbiological method of Snell and Wright (Abst.Vol. 11) was used for estimating nicotinic acid, certain modifications being introduced to secure a greater range within which the responses were linear.

Lactobacillus arabinosus for which nicotinic acid is a specific growth factor was the organism used. The modifications of procedure included those introduced by Krehl et al.

(Abst. For the microbiological assay of vitamins B12 in drugs, foodstuffs, animal feed preparations and other materials. -pantothenate 1 mg Folic acid μg Nicotinic acid 2 mg Pyridoxal hydrochloride 4 mg Pyridoxine hydrochloride 4 mg Pyridoxamine hydrochloride μg Riboflavin 1 mg no matter whether the microorganisms are of natural.

Ind. Eng. Chem. Anal. All Publications/Website. OR SEARCH CITATIONS. Niacin, also known as nicotinic acid, is an organic compound and a form of vitamin B3, an essential human nutrient.

Niacin is obtained in the diet from a variety of whole and processed foods, with highest contents in fortified packaged foods, meat, poultry, red fish such as tuna and salmon, lesser amounts in nuts, legumes and seeds.

Niacin as a dietary supplement is used to treat pellagra, a. Barton-Wright EC. The microbiological assay of nicotinic acid in cereals and other products. Biochem J. ; 38 (4)– [PMC free article] [Google Scholar] Barton-Wright EC, Booth RG.

The assay of riboflavin in cereals and other products: 1. Microbiological assay. Fluorometric assay. Biochem J. Apr; 37 (1)– Lactic Acid Bacterium Nicotinic Acid Pantothenic Acid Microbiological Method Microbiological Assay These keywords were added by machine and not by the authors.

This process is experimental and the keywords may be updated as the learning algorithm improves. The procedure followed in the improvement of the assay method was to use extracts of natural materials in the basal medium provided for the growth of Lactobacillus arabinosus.

These extracts were rendered nicotinic acid-free by adsorption of Llyod's reagent. Of the materials investigated, yeast and peptone were included in the medium. Nicotinic acid: Analogs and coenzymes; and derivatives; Section IX. Pteridines, analogs, and pterin coenzymes; Receive an update when the latest chapters in this book series are published.

Sign in to set up alerts. select article Contributors to volume XVIII, part b select article [] Microbiological assay of folic acid activity in.

biological assay is similar to that described by Snell and Wright (1) for the determination of nicotinic acid. A suitable mixture of pure amino acids is used to replace the casein hydrolysate in the medium for nicotinic acid assay.

p-Aminobenzoic acid and also a concentrate prepared from. Microbiological assay of nicotinic acid The pH is adjusted tousing bromo-thymol blue as external indicator and the volume made up to ml.; this is sufficient for 20 assay tubes.

A suitable quantity of this medium is prepared the day before experiment and stored overnight at Ibo. It is advisable to check the pH immediately before the experiment.Nicotinic acid, also known as niacin or vitamin B3, is a water-soluble vitamin whose derivatives such as NADH, NAD, NAD+, and NADP play essential roles in energy metabolism in the living cell and DNA repair.

The designation vitamin B3 also includes the amide form, nicotinamide or lack of niacin causes the deficiency disease pellagra, whereas a mild deficiency slows down the.nicotinamide in natural materials, although it is stated that “free nicotinic acid apparently does not occur in the living organism but is found in the urine of animals” (6).

The significance of nicotinamide in natural materials is further indicated by the fact that it is contained in ccenzymes I and II.